Part:BBa_K4727003:Design
M13 standard phagemid
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 623
Illegal suffix found at 645 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 623
Illegal SpeI site found at 646
Illegal PstI site found at 660
Illegal NotI site found at 629
Illegal NotI site found at 653 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 623 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 623
Illegal suffix found at 646 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 623
Plasmid lacks a suffix.
Illegal XbaI site found at 638
Illegal SpeI site found at 646
Illegal PstI site found at 660
Illegal NgoMIV site found at 127 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2026
Illegal SapI site found at 943
Design Notes
The design of this part started from the commercially available pTZ19R (ThermoFisher scientific). While this construct exhibited several favorable attributes aligned with our objectives, certain notable issues necessitated resolution. Primarily, there existed an obstacle due to the presence of a multiple cloning site embedded within the LacZ gene. This site encompassed three restricted cut sites, a circumstance that could be readily rectified through targeted deletion, achieved using a primer pair that excludes this region. Another significant concern was the absence of the BioBrick RFC[10] prefix and suffix. This challenge was similarly addressed straightforwardly, utilizing PCR mutagenesis to seamlessly incorporate the two required sequences.
Source
Deirived from pTZ19R by ThermoFischer scientific, a pUC19 plasmid containig the phage f1 intergenic region anche the T7 promoter